PAcrAm-g-(PEG, Amine, Silane)

PAcrAm-g-(PEG, amine, silane) is a random graft co-polymer with a poly(acrylamide) backbone, on which different side-chains have been reacted: poly(ethylene glycol) to impart non-fouling properties, and a combination of amine and silane binding groups for strong adherence onto silicon oxide and glass substrates. The mixture of the two (reversible and irreversible) binding groups enables a simple dip-and-rinse water-based coating procedure, as well as offering a unique coating stability in the presence of high salt concentrations, and high and low pH conditions, and commonly used surfactants. This enables the coatings to maintain their non-fouling character throughout the entire lifetime of the substrate.

Coating description: Poly(acrylamide)-graft-(PEG, 1,6-hexanediamine, 3-aminopropyldimethylethoxysilane) monomolecular coatings

Substrate examples: Glass, quartz, silicon wafers

Immobilization mechanism: Electrostatic adsorption and covalent linkage


Key product features:

  • Reduces adhesion of proteins, bacteria and cells onto surfaces
  • Simple dip-and-rinse coating procedure suitable for various substrates
  • Particularly strong bonding on glass and silicon oxide substrates
  • High coating stability in the presence of high salt concentrations, high and low pH and surfactants
  • Functionally equivalent, fluorescently tagged versions available (as standard products).
  • Easy to pattern
  • References: Serrano, A.; Zürcher, S.; Tosatti, S.; Spencer, N. D. Imparting Nonfouling Properties to Chemically Distinct Surfaces with a Single Adsorbing Polymer: A Multimodal Binding Approach. Macromol. Rapid Commun. 2016, 37 (7), 622–629 DOI: 10.1002/marc.201500683.

Typical applications:

  • Blocking of cell adhesion
  • Blocking of protein-nonspecific signals in microscopy applications
  • Non-reactive background for proteomics applications
  • Non-reactive background for cell-culture applications
  • Patterned biochips for controlled uptake of DNA/RNA strains, proteins, or cells