DBCO-reagents

DBCO (Dibenzocyclooctyne) reagents are used as labelling reagents that react exclusively and selectively with azide-tagged molecules or polymers such as PAcrAm™-g-(PMOXA-N3, NH2,X), PAcrAm™-g-(PMCA, NH2,X) or AQiBond™XX3T. They form a stable covalent bond upon spontaneous reaction with azide. This reaction, known as strain-promoted alkyne-azide cycloaddition (SPAAC), is fast and does not need additional catalysts or reagents. DBCO click chemistry can be run in aqueous buffers or organic solvents or mixtures thereof, depending on solubility and concentration of the reagents. Typical solution: 0.4 mg of the DBCO reagent dissolved in 5 mL of solvent to obtain a 0.1 µM solution.