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DBCO-reagents

DBCO reagents for SPAAC-clicking to azides decorating PAcrAm™-coated surfaces.

DBCO (Dibenzocyclooctyne) reagents are used as labeling reagents that react exclusively and selectively with azide-tagged molecules or polymers such as SuSoS PAcrAm™-g-(PMOXA-N3, NH2,X) and PAcrAm™-g-(PMCA, NH2,X). They form a stable covalent triazole ring upon a spontaneous reaction with azide. This reaction is known as strain promoted alkyne- azide cycloaddition (SPAAC), is fast and does not need additional catalysts or reagents.
DBCO click chemistry can be run in aqueous buffers or organic solvents or mixtures thereof, depending on solubility and concentration of the reagents.
SuSoS provides reagents in small aliquots ready to use for convenient surface modification experiments.

SuSoS currently offers:

  • DBCO-Biotin (DBCO-EG4-Biotin) to bind streptavidin, avidin, etc.
  • DBCO-NTA (DBCO-EG4-NTA) to reversibly immobilize of Histidine-tagged proteins
  • DBCO-RGD (RGD=CKK-(Acp)3- GRGDS) to selectively bind cells

 

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